Pryhuber_080318
- Project Type
- RNA-seq
- Library Preparation
- Clontech low input
- Sequencing Platform
- HiSeq 2500
- Data Formatting
- bcltofastq-2.19.0
- Data Cleaning
- Trimmomatic-0.36, TRAILING:13 LEADING:13 ILLUMINACLIP:adapters.fasta:2:30:10 SLIDINGWINDOW:4:20 MINLEN:35
- Genome Alignment
- STAR_2.6.0c, --twopassMode Basic --runMode alignReads --genomeDir ${GENOME} --readFilesIn ${SAMPLE} --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outFilterIntronMotifs RemoveNoncanonical
- Reference Genome
- GRCh38 + gencode28
- Read Quantification1
- subread-1.6.1, featurecounts, -s 0 -t exon -g gene_name
- Read Quantification2
- subread-1.6.1, featurecounts, -M -s 0 -t exon -g gene_name
Report generated on 2018-10-29, 09:43 based on data in:
/gpfs/fs2/scratch/grc_group/current_projects/Project_Pryhuber_080318
General Statistics
Showing 151/151 rows and 10/11 columns.Sample Name | M Raw Reads | % Dropped | % Dups | % GC | Length | M Seqs | % Aligned | M Aligned | % Assigned | M Assigned |
---|---|---|---|---|---|---|---|---|---|---|
D008-CBL-END-XPA-1 | 31.0 | 2.7% | 60.1% | 46% | 94 bp | 30.2 | 85.7% | 25.8 | 61.4% | 23.4 |
D008-CBL-EPI-XPA-1 | 31.3 | 3.0% | 58.7% | 46% | 94 bp | 30.3 | 86.3% | 26.2 | 62.5% | 22.1 |
D008-CBL-MES-XPA-1 | 32.8 | 2.6% | 55.2% | 46% | 95 bp | 32.0 | 91.2% | 29.1 | 70.4% | 25.0 |
D008-CBL-MIC-XPA-1 | 34.3 | 3.4% | 62.4% | 49% | 95 bp | 33.1 | 91.8% | 30.4 | 73.3% | 28.0 |
D008-CBL-PMX-XPA-1 | 26.6 | 2.7% | 56.6% | 48% | 94 bp | 25.9 | 89.5% | 23.2 | 67.8% | 20.7 |
D008-RML-BPS-XPA-1 | 34.9 | 2.7% | 56.7% | 47% | 95 bp | 33.9 | 89.9% | 30.5 | 65.4% | 26.6 |
D011-CBL-END-XPA-1 | 29.0 | 2.6% | 59.4% | 45% | 94 bp | 28.2 | 84.3% | 23.8 | 60.0% | 21.5 |
D011-CBL-EPI-XPA-1 | 24.8 | 3.8% | 67.4% | 38% | 91 bp | 23.9 | 70.5% | 16.8 | 47.7% | 13.9 |
D011-CBL-MES-XPA-1 | 26.3 | 6.3% | 59.0% | 43% | 92 bp | 24.6 | 80.6% | 19.9 | 57.1% | 18.2 |
D011-CBL-MIC-XPA-1 | 30.8 | 2.5% | 63.4% | 48% | 95 bp | 30.1 | 88.8% | 26.7 | 66.2% | 24.6 |
D011-CBL-PMX-XPA-1 | 32.0 | 3.1% | 61.0% | 45% | 93 bp | 31.0 | 83.7% | 25.9 | 61.1% | 23.3 |
D011-RML-BPS-XPA-1 | 32.5 | 2.6% | 54.0% | 48% | 95 bp | 31.7 | 90.8% | 28.8 | 64.7% | 24.2 |
D018-BRR-PMX-XPA-1 | 27.1 | 2.9% | 51.7% | 48% | 95 bp | 26.3 | 91.9% | 24.2 | 75.7% | 23.0 |
D018-CBL-END-XPA-2 | 28.6 | 3.4% | 63.4% | 42% | 94 bp | 27.7 | 77.7% | 21.5 | 58.0% | 19.2 |
D018-CBL-EPI-XPA-1 | 27.2 | 4.9% | 81.5% | 34% | 94 bp | 25.9 | 61.1% | 15.8 | 50.0% | 15.5 |
D018-CBL-MES-XPA-2 | 31.7 | 3.3% | 63.2% | 45% | 94 bp | 30.6 | 82.5% | 25.3 | 58.3% | 23.0 |
D018-CBL-MIC-XPA-2 | 26.8 | 3.2% | 65.9% | 45% | 94 bp | 26.0 | 82.4% | 21.4 | 61.5% | 20.1 |
D018-CBL-PMX-XPA-2 | 31.0 | 2.8% | 61.9% | 47% | 96 bp | 30.1 | 89.1% | 26.8 | 71.7% | 25.1 |
D018-RML-BPS-XPA-3 | 31.3 | 3.1% | 55.8% | 47% | 94 bp | 30.4 | 85.5% | 26.0 | 49.6% | 20.7 |
D019-BRR-PMX-XPA-1 | 31.8 | 3.3% | 61.0% | 44% | 94 bp | 30.7 | 83.7% | 25.7 | 65.5% | 23.5 |
D019-CBL-END-XPA-1 | 26.2 | 3.0% | 58.5% | 44% | 95 bp | 25.5 | 87.3% | 22.2 | 72.5% | 20.7 |
D019-CBL-EPI-XPA-1 | 25.6 | 2.8% | 60.3% | 44% | 94 bp | 24.9 | 87.7% | 21.8 | 70.1% | 19.9 |
D019-CBL-MES-XPA-1 | 30.4 | 3.5% | 58.7% | 43% | 94 bp | 29.4 | 80.9% | 23.8 | 57.6% | 20.8 |
D019-CBL-MIC-XPA-1 | 31.6 | 3.5% | 74.4% | 42% | 95 bp | 30.4 | 78.4% | 23.9 | 53.3% | 21.7 |
D019-CBL-PMX-XPA-1 | 25.9 | 3.0% | 55.4% | 46% | 95 bp | 25.1 | 89.3% | 22.4 | 66.5% | 20.3 |
D019-RML-BPS-XPA-1 | 39.9 | 3.2% | 53.7% | 47% | 96 bp | 38.6 | 88.2% | 34.0 | 57.6% | 28.7 |
D022-BRR-PMX-XPA-1 | 46.1 | 3.1% | 68.9% | 48% | 95 bp | 44.6 | 89.0% | 39.7 | 70.0% | 38.3 |
D022-CBL-END-XPA-1 | 25.6 | 3.1% | 55.1% | 47% | 95 bp | 24.8 | 90.9% | 22.5 | 68.2% | 19.8 |
D022-CBL-EPI-XPA-1 | 27.9 | 3.7% | 65.9% | 43% | 95 bp | 26.8 | 84.9% | 22.8 | 71.8% | 21.4 |
D022-CBL-MES-XPA-1 | 27.7 | 3.5% | 61.7% | 43% | 93 bp | 26.7 | 80.7% | 21.6 | 55.0% | 18.4 |
D022-CBL-MIC-XPA-1 | 27.8 | 3.7% | 64.4% | 47% | 93 bp | 26.8 | 86.8% | 23.2 | 60.0% | 19.6 |
D022-CBL-PMX-XPA-1 | 29.9 | 3.1% | 64.3% | 47% | 94 bp | 29.0 | 85.9% | 24.9 | 62.1% | 22.6 |
D022-RML-BPS-XPA-3 | 25.1 | 3.1% | 52.2% | 45% | 92 bp | 24.3 | 82.3% | 20.0 | 42.7% | 15.0 |
D024-BRR-PMX-XPA-1 | 38.8 | 4.1% | 63.3% | 47% | 95 bp | 37.3 | 88.1% | 32.8 | 68.5% | 31.1 |
D024-CBL-END-XPA-2 | 40.3 | 4.2% | 63.4% | 47% | 95 bp | 38.6 | 91.0% | 35.2 | 70.6% | 32.3 |
D024-CBL-EPI-XPA-2 | 25.3 | 4.1% | 58.4% | 44% | 93 bp | 24.3 | 84.8% | 20.6 | 63.1% | 18.3 |
D024-CBL-MES-XPA-2 | 40.8 | 4.0% | 64.4% | 47% | 95 bp | 39.1 | 88.4% | 34.6 | 69.6% | 32.5 |
D024-CBL-MIC-XPA-2 | 35.0 | 4.3% | 63.8% | 48% | 95 bp | 33.5 | 90.9% | 30.5 | 72.2% | 28.7 |
D024-CBL-PMX-XPA-2 | 34.9 | 4.1% | 61.8% | 47% | 96 bp | 33.5 | 91.0% | 30.5 | 71.2% | 28.4 |
D024-RML-BPS-XPA-1 | 33.4 | 5.2% | 49.2% | 47% | 94 bp | 31.7 | 89.4% | 28.3 | 54.8% | 21.1 |
D031_CBL_END_XPA_2 | 21.0 | 3.2% | 50.7% | 47% | 95 bp | 20.4 | 86.1% | 17.5 | 59.5% | 15.9 |
D031_CBL_EPI_XPA_2 | 22.0 | 3.6% | 77.5% | 41% | 93 bp | 21.2 | 79.5% | 16.9 | 64.3% | 17.5 |
D031_CBL_MES_XPA_2 | 19.7 | 3.1% | 47.9% | 46% | 95 bp | 19.1 | 86.3% | 16.5 | 56.7% | 14.0 |
D031_CBL_MIC_XPA_2 | 25.6 | 2.9% | 57.6% | 49% | 96 bp | 24.8 | 87.5% | 21.7 | 58.2% | 20.4 |
D031_CBL_PMX_XPA_2 | 20.1 | 3.0% | 54.7% | 47% | 94 bp | 19.5 | 83.9% | 16.4 | 57.5% | 15.5 |
D036-BRR-PMX-XPA-1 | 26.2 | 2.5% | 74.2% | 47% | 94 bp | 25.5 | 83.6% | 21.3 | 61.6% | 20.8 |
D036-CBL-END-XPA-2 | 24.7 | 2.5% | 62.9% | 41% | 94 bp | 24.1 | 76.5% | 18.4 | 54.3% | 16.0 |
D036-CBL-EPI-XPA-2 | 25.6 | 2.2% | 75.3% | 41% | 95 bp | 25.0 | 79.5% | 19.9 | 68.5% | 19.0 |
D036-CBL-MES-XPA-2 | 26.3 | 2.2% | 63.8% | 43% | 94 bp | 25.7 | 78.9% | 20.3 | 55.5% | 17.8 |
D036-CBL-MIC-XPA-2 | 24.3 | 3.2% | 61.0% | 49% | 95 bp | 23.5 | 91.7% | 21.5 | 71.8% | 19.8 |
D036-CBL-PMX-XPA-2 | 23.2 | 2.2% | 60.5% | 47% | 94 bp | 22.7 | 84.6% | 19.2 | 61.2% | 17.0 |
D038-BRR-PMX-XPA-1 | 22.3 | 3.2% | 67.1% | 44% | 93 bp | 21.6 | 80.9% | 17.5 | 59.0% | 16.4 |
D038-CBL-END-XPA-1 | 21.6 | 2.6% | 56.0% | 43% | 95 bp | 21.0 | 81.0% | 17.0 | 57.4% | 14.1 |
D038-CBL-EPI-XPA-1 | 22.7 | 2.5% | 59.0% | 44% | 94 bp | 22.1 | 81.6% | 18.0 | 54.0% | 15.5 |
D038-CBL-MES-XPA-1 | 21.3 | 2.3% | 49.5% | 47% | 95 bp | 20.8 | 88.7% | 18.4 | 62.3% | 15.7 |
D038-CBL-MIC-XPA-1 | 22.2 | 2.4% | 58.3% | 46% | 95 bp | 21.7 | 84.9% | 18.4 | 63.4% | 16.1 |
D038-CBL-PMX-XPA-1 | 22.5 | 3.2% | 63.5% | 41% | 94 bp | 21.8 | 73.4% | 16.0 | 53.0% | 14.0 |
D038-RML-BPS-XPA-1 | 21.9 | 2.4% | 48.2% | 45% | 93 bp | 21.4 | 84.6% | 18.1 | 54.9% | 14.8 |
D041-BRR-PMX-XPA-1 | 24.4 | 2.5% | 64.0% | 45% | 94 bp | 23.8 | 80.5% | 19.2 | 56.8% | 18.5 |
D041-CBL-END-XPA-1 | 21.3 | 2.6% | 51.8% | 47% | 96 bp | 20.8 | 89.8% | 18.7 | 64.4% | 15.8 |
D041-CBL-EPI-XPA-1 | 24.8 | 3.8% | 66.0% | 36% | 90 bp | 23.9 | 64.7% | 15.4 | 37.7% | 12.1 |
D041-CBL-MES-XPA-1 | 25.5 | 2.2% | 49.8% | 47% | 95 bp | 24.9 | 90.6% | 22.6 | 65.3% | 19.3 |
D041-CBL-MIC-XPA-1 | 23.2 | 2.3% | 58.4% | 49% | 96 bp | 22.7 | 90.5% | 20.5 | 69.5% | 18.7 |
D041-CBL-PMX-XPA-1 | 29.3 | 12.5% | 44.9% | 47% | 88 bp | 25.6 | 90.9% | 23.3 | 67.9% | 20.3 |
D041-RML-BPS-XPA-1 | 20.5 | 2.3% | 55.3% | 49% | 96 bp | 20.0 | 88.6% | 17.8 | 62.3% | 15.6 |
D043-BRR-PMX-XPA-1 | 23.7 | 2.8% | 71.0% | 47% | 94 bp | 23.0 | 83.8% | 19.3 | 61.5% | 18.4 |
D043-CBL-END-XPA-1 | 24.7 | 2.8% | 57.7% | 44% | 94 bp | 24.1 | 81.9% | 19.7 | 55.8% | 16.4 |
D043-CBL-EPI-XPA-1 | 24.2 | 5.1% | 72.8% | 43% | 93 bp | 22.9 | 84.8% | 19.4 | 72.6% | 19.7 |
D043-CBL-MES-XPA-1 | 24.8 | 2.9% | 64.7% | 43% | 94 bp | 24.1 | 80.2% | 19.3 | 58.7% | 17.0 |
D043-CBL-MIC-XPA-1 | 23.9 | 2.8% | 66.4% | 48% | 95 bp | 23.2 | 88.6% | 20.6 | 66.2% | 18.8 |
D043-CBL-PMX-XPA-1 | 25.0 | 2.5% | 61.5% | 46% | 94 bp | 24.4 | 85.5% | 20.8 | 64.0% | 19.0 |
D043-RML-BPS-XPA-1 | 22.8 | 2.6% | 51.6% | 45% | 93 bp | 22.2 | 84.1% | 18.7 | 57.0% | 15.9 |
D044-BRR-PMX-XPA-1 | 20.7 | 2.6% | 62.3% | 47% | 94 bp | 20.2 | 84.8% | 17.1 | 62.4% | 16.5 |
D044-CBL-END-XPA-1 | 24.2 | 5.5% | 52.9% | 47% | 92 bp | 22.9 | 89.7% | 20.5 | 67.8% | 18.0 |
D044-CBL-EPI-XPA-1 | 26.4 | 2.5% | 54.6% | 46% | 94 bp | 25.7 | 89.7% | 23.1 | 67.6% | 20.4 |
D044-CBL-MES-XPA-1 | 24.2 | 2.7% | 54.1% | 46% | 94 bp | 23.5 | 89.8% | 21.1 | 64.6% | 17.5 |
D044-CBL-MIC-XPA-1 | 34.0 | 2.6% | 63.1% | 47% | 95 bp | 33.1 | 88.3% | 29.2 | 61.7% | 25.1 |
D044-CBL-PMX-XPA-1 | 32.0 | 3.2% | 57.5% | 47% | 95 bp | 30.9 | 90.2% | 27.9 | 68.9% | 25.0 |
D044-RML-BPS-XPA-1 | 37.5 | 3.0% | 55.2% | 46% | 95 bp | 36.3 | 87.0% | 31.6 | 53.0% | 25.3 |
D056_CBL_END_XPA_2 | 9.7 | 26.9% | 29.8% | 45% | 84 bp | 7.1 | 85.3% | 6.1 | 52.4% | 5.0 |
D056_CBL_EPI_XPA_2 | 21.5 | 2.9% | 72.3% | 45% | 94 bp | 20.9 | 87.9% | 18.4 | 71.9% | 18.7 |
D056_CBL_MES_XPA_2 | 19.5 | 3.8% | 58.9% | 46% | 94 bp | 18.7 | 84.7% | 15.9 | 60.5% | 15.2 |
D056_CBL_MIC_XPA_2 | 23.8 | 3.3% | 61.0% | 50% | 95 bp | 23.0 | 87.2% | 20.0 | 58.9% | 19.1 |
D056_CBL_PMX_XPA_2 | 22.4 | 2.9% | 62.5% | 49% | 95 bp | 21.7 | 86.8% | 18.9 | 59.8% | 18.1 |
D063_CBL_END_XPA_1 | 23.8 | 2.8% | 51.5% | 48% | 95 bp | 23.2 | 87.6% | 20.3 | 57.1% | 17.5 |
D063_CBL_EPI_XPA_1 | 20.8 | 3.3% | 78.6% | 41% | 94 bp | 20.1 | 75.9% | 15.3 | 65.0% | 15.3 |
D063_CBL_MES_XPA_1 | 24.0 | 2.9% | 55.5% | 46% | 95 bp | 23.3 | 87.4% | 20.3 | 66.3% | 18.1 |
D063_CBL_MIC_XPA_1 | 19.5 | 2.8% | 57.7% | 50% | 95 bp | 18.9 | 87.2% | 16.5 | 58.5% | 15.7 |
D063_CBL_PMX_XPA_1 | 20.5 | 2.9% | 58.1% | 48% | 95 bp | 19.9 | 86.6% | 17.2 | 59.5% | 16.3 |
D071-BRR-PMX-XPA-1 | 19.4 | 2.1% | 52.9% | 47% | 95 bp | 19.0 | 86.6% | 16.5 | 61.7% | 14.7 |
D071-RML-BPS-XPA-1 | 19.6 | 1.8% | 48.1% | 47% | 96 bp | 19.2 | 88.8% | 17.1 | 58.4% | 14.3 |
D071-RML-END-XPA-1 | 21.6 | 2.0% | 52.9% | 48% | 95 bp | 21.1 | 89.4% | 18.9 | 64.9% | 16.7 |
D071-RML-EPI-XPA-1 | 22.5 | 23.8% | 47.2% | 47% | 84 bp | 17.1 | 86.6% | 14.8 | 68.2% | 14.6 |
D071-RML-MES-XPA-1 | 22.1 | 2.1% | 60.9% | 47% | 94 bp | 21.7 | 85.8% | 18.6 | 63.4% | 18.0 |
D071-RML-MIC-XPA-1 | 21.8 | 3.1% | 73.8% | 47% | 94 bp | 21.1 | 83.7% | 17.7 | 61.0% | 16.5 |
D071-RML-PMX-XPA-1 | 18.7 | 1.9% | 59.8% | 49% | 96 bp | 18.3 | 89.5% | 16.4 | 71.4% | 15.5 |
D075_CBL_END_XPA_1 | 23.7 | 3.2% | 51.1% | 48% | 96 bp | 22.9 | 91.1% | 20.9 | 67.0% | 19.2 |
D075_CBL_EPI_XPA_1 | 22.3 | 3.3% | 64.9% | 47% | 95 bp | 21.6 | 88.1% | 19.0 | 70.0% | 18.9 |
D075_CBL_MES_XPA_1 | 21.3 | 4.8% | 45.4% | 48% | 94 bp | 20.3 | 89.7% | 18.2 | 61.7% | 15.9 |
D075_CBL_MIC_XPA_1 | 20.7 | 3.3% | 55.8% | 50% | 95 bp | 20.0 | 89.1% | 17.9 | 62.7% | 16.7 |
D075_CBL_PMX_XPA_1 | 22.9 | 3.4% | 50.8% | 48% | 95 bp | 22.1 | 89.2% | 19.7 | 63.4% | 17.9 |
D092_CBL_END_XPA_1 | 23.9 | 3.0% | 53.6% | 48% | 96 bp | 23.2 | 90.3% | 20.9 | 65.0% | 19.0 |
D092_CBL_EPI_XPA_1 | 25.1 | 3.5% | 72.7% | 45% | 94 bp | 24.3 | 85.0% | 20.6 | 70.5% | 21.4 |
D092_CBL_MES_XPA_1 | 19.8 | 2.8% | 49.4% | 48% | 96 bp | 19.2 | 90.0% | 17.3 | 62.4% | 15.4 |
D092_CBL_MIC_XPA_1 | 21.3 | 3.1% | 60.6% | 50% | 96 bp | 20.7 | 88.7% | 18.3 | 62.9% | 17.5 |
D092_CBL_PMX_XPA_1 | 21.5 | 2.9% | 56.8% | 49% | 96 bp | 20.9 | 88.8% | 18.5 | 63.9% | 17.5 |
D105_CBL_END_XPA_2 | 20.7 | 2.8% | 45.1% | 48% | 95 bp | 20.1 | 90.0% | 18.1 | 59.4% | 15.3 |
D105_CBL_EPI_XPA_2 | 15.6 | 10.1% | 68.9% | 43% | 90 bp | 14.0 | 85.2% | 11.9 | 71.2% | 12.1 |
D105_CBL_MES_XPA_2 | 20.4 | 3.5% | 44.9% | 48% | 95 bp | 19.7 | 88.7% | 17.5 | 59.8% | 15.3 |
D105_CBL_MIC_XPA_2 | 21.7 | 3.1% | 56.2% | 50% | 95 bp | 21.0 | 87.5% | 18.3 | 58.5% | 17.3 |
D105_CBL_PMX_XPA_2 | 17.8 | 3.0% | 50.9% | 49% | 95 bp | 17.3 | 88.2% | 15.2 | 61.2% | 14.4 |
D110_CBL_END_XPA_1 | 19.5 | 2.8% | 53.0% | 49% | 96 bp | 19.0 | 90.1% | 17.1 | 64.2% | 16.2 |
D110_CBL_EPI_XPA_1 | 22.6 | 3.7% | 63.0% | 50% | 95 bp | 21.8 | 88.7% | 19.3 | 66.4% | 19.1 |
D110_CBL_MES_XPA_1 | 21.1 | 3.0% | 47.2% | 48% | 95 bp | 20.4 | 88.2% | 18.0 | 56.1% | 15.7 |
D110_CBL_MIC_XPA_1 | 27.0 | 3.0% | 61.3% | 50% | 95 bp | 26.2 | 88.0% | 23.1 | 60.6% | 22.3 |
D110_CBL_PMX_XPA_1 | 23.5 | 3.0% | 56.0% | 49% | 95 bp | 22.8 | 88.7% | 20.2 | 63.4% | 19.5 |
D113_CBL_END_XPA_1 | 19.3 | 4.7% | 48.8% | 48% | 95 bp | 18.4 | 90.6% | 16.7 | 68.1% | 15.8 |
D113_CBL_EPI_XPA_1 | 23.0 | 3.3% | 58.4% | 50% | 95 bp | 22.2 | 89.7% | 20.0 | 68.9% | 19.4 |
D113_CBL_MES_XPA_1 | 22.1 | 3.5% | 44.4% | 48% | 95 bp | 21.3 | 90.5% | 19.3 | 61.4% | 16.4 |
D113_CBL_MIC_XPA_1 | 21.2 | 3.3% | 56.9% | 50% | 96 bp | 20.5 | 90.1% | 18.5 | 67.0% | 17.8 |
D113_CBL_PMX_XPA_1 | 24.9 | 3.6% | 51.9% | 50% | 95 bp | 24.0 | 90.0% | 21.6 | 66.9% | 20.4 |
D116_RML_END_XPA_2 | 20.1 | 2.9% | 41.8% | 46% | 94 bp | 19.5 | 87.4% | 17.1 | 48.0% | 12.1 |
D116_RML_EPI_XPA_2 | 22.4 | 3.2% | 69.0% | 44% | 94 bp | 21.7 | 84.6% | 18.4 | 65.0% | 17.8 |
D116_RML_MES_XPA_2 | 23.7 | 3.1% | 56.5% | 47% | 95 bp | 23.0 | 85.0% | 19.5 | 57.5% | 18.0 |
D116_RML_MIC_XPA_2 | 20.1 | 3.0% | 59.4% | 50% | 95 bp | 19.5 | 87.0% | 16.9 | 59.0% | 16.4 |
D116_RML_PMX_XPA_2 | 21.1 | 3.2% | 60.0% | 50% | 95 bp | 20.4 | 86.4% | 17.6 | 58.3% | 17.0 |
D119_CBL_END_XPA_1 | 23.1 | 3.2% | 55.5% | 48% | 95 bp | 22.4 | 88.4% | 19.8 | 61.8% | 18.5 |
D119_CBL_EPI_XPA_1 | 20.3 | 3.2% | 61.8% | 47% | 95 bp | 19.7 | 88.7% | 17.5 | 67.6% | 16.8 |
D119_CBL_MES_XPA_1 | 22.0 | 3.5% | 46.7% | 48% | 95 bp | 21.2 | 89.0% | 18.9 | 58.1% | 16.1 |
D119_CBL_MIC_XPA_1 | 18.7 | 4.0% | 53.4% | 49% | 95 bp | 18.0 | 86.7% | 15.6 | 56.4% | 14.5 |
D119_CBL_PMX_XPA_1 | 19.9 | 4.8% | 47.9% | 48% | 94 bp | 18.9 | 88.3% | 16.7 | 59.5% | 15.0 |
D133_CBL_END_XPA_1 | 23.4 | 3.2% | 49.0% | 46% | 95 bp | 22.6 | 87.8% | 19.9 | 58.9% | 16.1 |
D133_CBL_EPI_XPA_1 | 24.4 | 3.6% | 81.1% | 42% | 94 bp | 23.5 | 82.5% | 19.4 | 75.3% | 20.2 |
D133_CBL_MES_XPA_1 | 19.5 | 3.7% | 48.5% | 46% | 95 bp | 18.7 | 86.9% | 16.3 | 60.3% | 14.1 |
D133_CBL_MIC_XPA_1 | 23.0 | 3.2% | 54.2% | 49% | 95 bp | 22.2 | 87.9% | 19.5 | 56.2% | 16.8 |
D133_CBL_PMX_XPA_1 | 18.7 | 3.3% | 55.1% | 48% | 95 bp | 18.1 | 86.9% | 15.7 | 59.8% | 14.3 |
D134_CBL_END_XPA_1 | 20.4 | 3.3% | 49.9% | 43% | 94 bp | 19.7 | 79.6% | 15.7 | 44.2% | 11.2 |
D134_CBL_EPI_XPA_1 | 18.9 | 3.2% | 79.2% | 41% | 94 bp | 18.3 | 83.7% | 15.4 | 73.2% | 15.4 |
D134_CBL_MES_XPA_1 | 15.0 | 9.8% | 58.8% | 41% | 91 bp | 13.6 | 78.0% | 10.6 | 54.3% | 9.6 |
D134_CBL_MIC_XPA_1 | 19.0 | 3.0% | 53.2% | 49% | 95 bp | 18.4 | 88.4% | 16.3 | 58.5% | 14.6 |
D134_CBL_PMX_XPA_1 | 18.9 | 2.9% | 55.5% | 48% | 96 bp | 18.4 | 87.9% | 16.2 | 61.0% | 14.7 |
D136_CBL_END_XPA_1 | 17.6 | 5.9% | 44.8% | 46% | 93 bp | 16.6 | 88.4% | 14.7 | 57.5% | 12.4 |
D136_CBL_EPI_XPA_1 | 16.1 | 3.0% | 62.3% | 43% | 94 bp | 15.6 | 82.8% | 12.9 | 55.6% | 10.8 |
D136_CBL_MES_XPA_1 | 20.0 | 2.8% | 47.0% | 47% | 95 bp | 19.4 | 86.9% | 16.9 | 53.5% | 14.0 |
D136_CBL_MIC_XPA_1 | 21.1 | 2.6% | 49.6% | 48% | 95 bp | 20.6 | 88.2% | 18.1 | 54.4% | 15.2 |
D136_CBL_PMX_XPA_1 | 10.1 | 29.8% | 31.0% | 46% | 84 bp | 7.1 | 86.2% | 6.1 | 57.6% | 5.7 |
D143_CBL_END_XPA_1 | 21.9 | 3.7% | 53.2% | 49% | 95 bp | 21.1 | 91.3% | 19.3 | 68.9% | 18.4 |
D143_CBL_EPI_XPA_1 | 17.1 | 4.0% | 54.7% | 48% | 94 bp | 16.4 | 88.7% | 14.6 | 67.6% | 14.2 |
D143_CBL_MES_XPA_1 | 21.7 | 2.9% | 50.0% | 48% | 96 bp | 21.1 | 90.4% | 19.1 | 63.8% | 17.4 |
D143_CBL_MIC_XPA_1 | 15.6 | 12.4% | 49.0% | 49% | 90 bp | 13.7 | 87.9% | 12.0 | 63.0% | 11.7 |
D143_CBL_PMX_XPA_1 | 21.2 | 3.2% | 53.3% | 49% | 95 bp | 20.5 | 90.2% | 18.5 | 67.6% | 17.8 |
Trimmomatic
Trimmomatic is a flexible read trimming tool for Illumina NGS data.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
FastQC
FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.
Sequence Quality Histograms
The mean quality value across each base position in the read. See the FastQC help.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Per Sequence Quality Scores
The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Per Base Sequence Content
The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.
Rollover for sample name
Per Sequence GC Content
The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Per Base N Content
The percentage of base calls at each position for which an N was called. See the FastQC help.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Sequence Length Distribution
The distribution of fragment sizes (read lengths) found. See the FastQC help.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Sequence Duplication Levels
The relative level of duplication found for every sequence. See the FastQC help.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Overrepresented sequences
The total amount of overrepresented sequences found in each library. See the FastQC help for further information.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Adapter Content
The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
STAR
STAR is an ultrafast universal RNA-seq aligner.
Alignment Scores
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
FeatureCounts (Unique Reads)
Subread featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
FeatureCounts (Multi Mapping Reads)
Subread featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).